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An oxidation pathway, in which jasmonoyl-isoleucine is oxidized by cytochrome P450 enzymes, mainly CYP94 isoforms, to a 12-hydroxy metabolite and then further via a 12aldehydo intermediate to dicarboxy-jasmonoyl-isoleucine, together with deconjugation by amidohydrolases, is now recognized as a major route for catabolism and deactivation of the hormone, although the hydroxy intermediate can itself cause weak and sustainable expression of certain JA-responsive genes until further oxidation occurs

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